- ￥1350 - 3000
- Proteintech
- 66963-1-Ig
- 2025年10月13日
- WB, IHC, ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NFATC1 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, ELISA
- 靶点：
NFATC1
- 抗体英文名：
NFATC1 Monoclonal antibody
- 抗体名：
NFATC1 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, U2OS cells, Daudi cells, Romas cells, Raji cells, A549 cells, MOLT-4 cells, Ramos cells, Karpas-422 cells |
| Positive IHC detected in | human urothelial carcinoma tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66963-1-Ig targets NFATC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag13014 Product name: Recombinant human NFATC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC104753 Sequence: MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSWLGNTTQYTSSAIVAAINALTTDSSLDLGDGVPVKSRKTTLEQPPSVALKVE |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 |
| 别名 | 1E1B10, NFAT2, NFATc, NF-ATc, NF-ATc1 |
| 计算分子量 | 716aa,78 kDa; 943aa,101 kDa |
| 观测分子量 | 101 kDa |
| GenBank蛋白编号 | BC104753 |
| 基因名称 | NFATC1 |
| Gene ID (NCBI) | 4772 |
| RRID | AB_2882286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O95644 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Acta Pharm Sin B KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancerAuthors - Cancan Zheng |
| human | WB | Mol Ther C5a-C5aR1 axis controls mitochondrial fission to promote podocyte injury in lupus nephritisAuthors - Baokui Ye |
| human | WB,IF | Pharmacol Res Targeting CD38/ ADP-ribosyl cyclase as a novel therapeutic strategy for identification of three potent agonists for leukopenia treatmentAuthors - Yuanzhi Liu |
| rat | WB,IP,IF | Cell Mol Biol Lett The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesisAuthors - Tao Yang |
| human | WB | Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.Authors - Mengyi Lao |
| human | WB,IF | Cancer Lett TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.Authors - Da-Cen Lin |
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后，B细胞转化为浆细胞，由浆细胞产生与抗原发生特异性结合的球蛋白，这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类：IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
浓度很高。可见该法操作简便、经济，不过，腹水中常混有小鼠的各种杂蛋白（包括Ig），因此在很多情况下要提纯后才能使用，而且还有污染动物病毒的危险，故而最好用SPF级小鼠。（1）材料：a. 成年BALB/c小鼠。b. 降植烷（Pristane）或液体石蜡。c. 处于对数生长期的杂交瘤细胞。（2）方法：a. 腹腔接种降植烷或液体石蜡，每只小鼠0.3-0.5ml。b. 7-10天后腹腔接种用PBS或无血清培养基稀释的杂交瘤细胞，每只小鼠5×105/0.2ml。c. 间隔5天后，每天观察小鼠腹水产生情况，如腹部
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