- ￥1500 - 3280
- Proteintech
- 66854-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Mouse
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
Phospho-RIPK1 (Ser161)
- 抗体英文名：
Phospho-RIPK1 (Ser161) Monoclonal antibody
- 抗体名：
Phospho-RIPK1 (Ser161) Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells |
| Positive IHC detected in | human small intestine tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000- 1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| 别名 | p RIPK1, p RIPK1 (Ser161), p RIPK1 Ser161, Phospho RIPK1, Phospho RIPK1 (Ser161) |
| 计算分子量 | 76 kDa |
| 观测分子量 | 74 kDa, 45 kDa |
| GenBank蛋白编号 | NM_003804 |
| 基因名称 | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_2882194 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13546 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cancer Lett NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathwayAuthors - Jinghan Zhu |
| mouse | WB,IHC,IF | Cell Commun Signal IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritisAuthors - Kai Sun |
| human | WB | Inflammation Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected MacrophagesAuthors - Jingjing Shen |
| human | WB | J Cell Mol Med LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.Authors - Chia-Kang Ho |
| mouse | WB | BMC Complement Med Ther JianPiYiShen formula prevents cisplatin-induced acute kidney injury in mice by improving necroptosis through MAPK pathwayAuthors - Zhongtang Li |
| human | WB | Front Genet Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.Authors - Yongchang Yang |
膜上有关；后者与抗原信号传递和Ca2+流动有关。其中的极性氨基酸(Thr、Ser)与抗原识别相关分子之间形成盐键，以稳定BCR复合结构。mlg的胞内区极短，mlgM和mlgD均只有3个氨基酸残基，与T细胞中的TCR相类似，BCR需要与Iga(CD79a)和Igp(CD79b)以非共价结合形式构成复合体，共同完成对抗原的识别及信号转导。 B细胞和T细胞抗原识别结构的主要成分比较 Igα和Igβ为单链I型跨膜蛋白，分子质量分别是33kDa和37kDa，以二硫
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
技术资料暂无技术资料 索取技术资料
