- ￥1350 - 3000
- Proteintech
- 66579-1-Ig
- 2025年10月13日
- WB, IF/ICC, ELISA
- Mouse
- Human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PRKAB2 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
AMPK Beta 2
- 抗体英文名：
AMPK Beta 2 Monoclonal antibody
- 抗体名：
AMPK Beta 2 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A375 cells, A431 cells, HeLa cells, A549 cells, human brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66579-1-Ig targets AMPK Beta 2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag5806 Product name: Recombinant human PRKAB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC053610 Sequence: MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, AMP-activated, beta 2 non-catalytic subunit |
| 别名 | AMPK subunit beta 2, PRKAB2 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC053610 |
| 基因名称 | AMPK Beta 2 |
| Gene ID (NCBI) | 5565 |
| RRID | AB_2881939 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43741 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.
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文献和实验CD79a Encodes the Ig-alpha protein of the B-cell antigen receptor; receptor also includes Ig-beta protein, surface immunoglobulin Expressed early in B-cell differentiation (often positive when mature B-cell markers are negative
子蛋白质结合后能引起机体产生特异性抗体的，称为半抗原(hapten)。例如某些激素、药物等。抗原的反应性取决于抗原决定簇(antigenic determinant)，或称为表位(epitope)。一个抗原分子可带有不同的决定簇。 1.2 抗体 1.2.1 抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白 (immunoglobulin,Ig)。Ig分五类，即IgG、IgA、IgM、IgD和IgE。与免疫测定有关的Ig主要为IgG和IgM。Ig由两个轻链(L
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