- ￥1350 - 3000
- Proteintech
- 66344-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ANXA1 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
Annexin A1
- 抗体英文名：
Annexin A1 Monoclonal antibody
- 抗体名：
Annexin A1 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, A431 cells, NIH/3T3 cells, 4T1 cells, HCT 116 cells, Jurkat cells, K-562 cells, HepG2 cells |
| Positive IHC detected in | human stomach tissue, human liver tissue, human pancreas tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66344-1-Ig targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17273 Product name: Recombinant human ANXA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC001275 Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | annexin A1 |
| 别名 | ANXA1, 1E1B7, Annexin 1, Annexin Ac2-26, Annexin I |
| 计算分子量 | 346 aa, 39 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC001275 |
| 基因名称 | Annexin A1 |
| Gene ID (NCBI) | 301 |
| RRID | AB_2881724 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04083 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Adv Sci (Weinh) M2 Macrophage-Derived Extracellular Vesicles Reprogram Immature Neutrophils into Anxa1hi Neutrophils to Enhance Inflamed Bone RegenerationAuthors - Yufei Yao |
| human | IF | Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.Authors - Emna Ouni |
| human | IHC | J Mater Chem B Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heatingAuthors - Bin Yan |
| human | IF | Nat Commun Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesisAuthors - Maria Fankhaenel |
| mouse | J Proteome Res Effect of Tamoxifen on Proteome Expression during In Vitro Myogenesis in Murine Skeletal Muscle C2C12 CellsAuthors - Emily A Morris | |
| human | IF | Nature Stress granules plug and stabilize damaged endolysosomal membranesAuthors - Claudio Bussi |
Annexin V-PI 双染法 在细胞凋亡的早期就有细胞膜表面破损发生。破损时凋亡细胞表面的磷脂腺丝氨酸(PS)可以从细胞内膜翻转至细胞的外膜。该过程发生在DNA片段化之前，因而检测PS的表达，能反映早期凋亡。AnnexinV是相对分子质量为35 000大小的Ca2+依赖的磷脂结合蛋白，对PS有很高的亲和性，并且可以与暴露于细胞外的PS相结合。利用这一原理，可以将AnnexinV标记荧光来识别早期的细胞凋亡。通常利用AnnexinV-FITC和碘化丙啶(P1)来区分凋亡和坏死细胞。PI
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后，B细胞转化为浆细胞，由浆细胞产生与抗原发生特异性结合的球蛋白，这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类：IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
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