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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IFNA1 protein
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
IFN Alpha
- 抗体英文名:
IFN Alpha Monoclonal antibody
- 抗体名:
IFN Alpha Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | Recombinant protein, Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66162-1-Ig targets IFN Alpha in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12533 Product name: Recombinant human IFNA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-189 aa of BC074928 Sequence: CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IFNA1 |
| 别名 | IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, IFN-alpha, LeIF D |
| 计算分子量 | 189 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC074928 |
| 基因名称 | IFN alpha1 |
| Gene ID (NCBI) | 3439 |
| RRID | AB_2881558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01562 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Amino Acids Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.Authors - Yan Jin |
| human | WB | Brain Res Serum miR-221-3p as a new potential biomarker for depressed mood in perioperative patients.Authors - Jianguo Feng |
| human | WB | Cell Rep Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesisAuthors - Juanjuan Luo |
Measuring Interferon Alpha and Other Cytokines in SLE
The progression of disease in patients with systemic lupus erythematosus (SLE) is affected by production, accumulation, and actions of cytokines. Type I interferon (IFN), specifically IFN-α, is recognized as a central mediator of disease
和FeγRⅢ三类,它们的结构和分布有所不同。 (1)FeγRⅠ(CD64):为70kD穿膜糖蛋白,属Ig超家族,胞膜外区有3个C2结构,FeγRⅠ主要分布于单核细胞、巨噬细胞、中性粒细胞等。IFN-γ可刺激单核细胞、巨噬细胞中性细胞表达FeγRⅠ水平增加5~10倍,G-CSF也这种促进作用。 (2)FeγRⅡ(CD32),为40kD穿膜糖蛋白,属于Ig超家族,胞膜外区有2个C2结构,FeγRⅡ表达于除红细胞外的其它血细胞。 (3)FeγRⅢ(CD16):为50~70kD糖
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
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