- ￥1350 - 3000
- Proteintech
- 60309-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Mouse
- human, mouse, rat, pig, rabbit, chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRAS protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit, chicken
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
pan Ras
- 抗体英文名：
pan Ras Monoclonal antibody
- 抗体名：
pan Ras Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | fetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60309-1-Ig targets pan Ras in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | KRAS, 4H4G7, C K RAS, c Ki ras, c-Ki-ras |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC013572 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2881422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Mol Cell TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.Authors - Huafeng Zhang |
| human | WB | Cancer Res KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.Authors - Peng Yuan
|
| human | IHC | Exp Mol Med Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.Authors - Won-Ji Ryu |
| human | WB | J Cell Mol Med DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.Authors - Wei Wang |
| human | WB | Mol Pharm Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast CancerAuthors - Xiaoxia Yang |
| human | WB | Oncol Rep EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.Authors - Chuan Lin |
PNAS：RAS 抑制剂又添一员，新型蛋白模拟物或为癌症治疗
-Sos 复合体结构为合理设计稳定的 Ras 抑制剂提供了依据。来自纽约大学的 Paramjit S. Arora 团队在 PNAS 在线发表了题为 A Sos proteomimetic as a pan-Ras inhibitor [1] 的文章。该研究开发了一种结构稳定的 Sos 蛋白模拟物，可与野生型和致癌型 Ras 非共价结合，并调节下游激酶信号。该模拟物能够抵抗蛋白水解并进入癌细胞，对巨胞饮作用上调的癌细胞具有选择性毒性，包括那些具有致癌 Ras 突变的细胞，为开发 Ras 突变型癌症非
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后，B细胞转化为浆细胞，由浆细胞产生与抗原发生特异性结合的球蛋白，这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类：IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
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