- ￥5800
- Proteintech
- 60309-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
- Mouse
- human, mouse, rat, pig, rabbit, chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRAS protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit, chicken
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
- 靶点：
pan Ras
- 抗体英文名：
pan Ras Monoclonal antibody, PBS Only (Capture/Detector)
- 抗体名：
pan Ras Monoclonal antibody, PBS Only (Capture/Detector)
- 规格：
100ug
产品信息
60309-1-PBS targets pan Ras as part of a matched antibody pair:
MP50398-1: 60309-2-PBS capture and 60309-1-PBS detection (validated in Cytometric bead array)
MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 免疫原 |
CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | KRAS, 4H4G7, C K RAS, c Ki ras, c-Ki-ras |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC013572 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2881422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
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文献和实验分钟。 (2)取50μl50mg/L的癌基因探针Y13-25（它是一种广谱单克隆抗体，可特异性地直接与N-ras，Ki-ras和Ha-ras三种癌基因编码的21Kd蛋白相结合），4℃作用30～45分钟。 (3)用PB离心洗涤2次，重悬浮于50μl HBSS中（含0.1%叠氮钠和2%小牛血清）。 (4)加入50μl 1：200兔抗鼠IgG，4℃放置30～45分钟。 (5)同(3)洗涤后加入50μl 1：40的FITC标记的羊抗兔IgG，4℃反应30～45分钟。 (6)同(3)洗涤后，细胞
Systematic Evaluation of Skeletal Mechanical Function
. Cylindrical tube fits securely into the three‐jaw chuck between the X‐ray source (right) and detector panel (left). In this system, the tube is stationary, the detector can shift to the right or left to allow a larger field of view, and the specimen rotates
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
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