- ￥1350 - 3000
- Proteintech
- 55027-1-AP
- 2025年10月13日
- WB, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
CSPG4
- 抗体英文名：
CSPG4 Polyclonal antibody
- 抗体名：
CSPG4 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55027-1-AP targets CSPG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chondroitin sulfate proteoglycan 4 |
| 别名 | NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP |
| 计算分子量 | 251 kDa |
| 观测分子量 | 240-250 kDa, 450kDa |
| GenBank蛋白编号 | NM_001897 |
| 基因名称 | CSPG4 |
| Gene ID (NCBI) | 1464 |
| RRID | AB_10791888 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UVK1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. CSPG4 functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It promotes retraction fiber formation and cell polarization through Rho GTPase activation and stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. CSPG4 activates FAK and ERK1/ERK2 signaling cascades. The antibody is specific to CSPG4.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.Authors - Steinberg Florian F |
| mouse | Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitmentAuthors - Zongchao Sun | |
| human | WB | J Clin Invest Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progressionAuthors - Shike Wang |
| mouse | WB | J Pineal Res Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.Authors - Lirong Liang |
| mouse | WB | Glia ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.Authors - Lukasz Mateusz Szewczyk |
| mouse | Commun Biol Complete remission of diabetes with a transient HDAC inhibitor and insulin in streptozotocin miceAuthors - Hideto Kojima |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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