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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
ATP6
- 抗体英文名:
ATP6 Polyclonal antibody
- 抗体名:
ATP6 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, C2C12 cells, rat brain |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55313-1-AP targets ATP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase 6; ATPase subunit 6 |
| 别名 | MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | J01415 |
| 基因名称 | ATP6 |
| Gene ID (NCBI) | 4508 |
| RRID | AB_2881305 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00846 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.Authors - Xinxin Zuo |
| human | WB | Nat Struct Mol Biol Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNAAuthors - R Stefan Isaac |
| human | WB | Mol Cell Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.Authors - Xiaoyu Li |
| human | WB | Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.Authors - Uwe Richter |
| human | WB | Nucleic Acids Res A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.Authors - Zidong Jia |
| human, mouse | WB | Nat Commun Deregulation of mitochondrial F1FO-ATP synthase via OSCP in Alzheimer's disease.Authors - Simon J Beck |
Nature 重磅:林圣彩院士团队七年攻坚!首次破解「神药」二甲双胍作用靶点之谜
)。 图片来源:Nature 因此,接下来,他们就使用了一种基于亲和力的方法来分析纯化的溶酶体蛋白提取物,以确定二甲双胍潜在的直接靶点。经过不厌其烦地逐一筛选,他们从 2000 多个获选靶点中锁定了 PEN2。他们发现 PEN2 的缺失使细胞对二甲双胍治疗不敏感。同样地,敲除 PEN2 也可阻断低剂量二甲双胍诱导的 AMPK 激活和 v-ATPase 抑制。 图片来源:Nature ATP6AP1 辅助 PEN2 与 v-ATPase 结合 接下来,他们研究了二甲双胍是如何导致 PEN
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
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