- ￥1350 - 3000
- Proteintech
- 28131-1-AP
- 2025年10月13日
- WB, IHC, IF-P, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SLC4A1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, ELISA
- 靶点：
Band 3/AE 1
- 抗体英文名：
Band 3/AE 1 Polyclonal antibody
- 抗体名：
Band 3/AE 1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse liver tissue |
| Positive IHC detected in | human spleen tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28131-1-AP targets Band 3/AE 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27878 Product name: Recombinant human SLC4A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC101570 Sequence: MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) |
| 别名 | band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1 |
| 计算分子量 | 911 aa, 102 kDa |
| 观测分子量 | 95-100 kDa |
| GenBank蛋白编号 | BC101570 |
| 基因名称 | band 3 |
| Gene ID (NCBI) | 6521 |
| RRID | AB_2881069 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02730 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Mol Cell The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.Authors - Eunus S Ali |
| human | IP | Int J Hematol Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.Authors - Akihito Kitao |
| mouse | IF | eLife Piezo1 as a force-through-membrane sensor in red blood cellsAuthors - George Vaisey |
| human | IF | NPJ Regen Med Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endotheliumAuthors - Soo-Been Jeon |
| mouse | IF | Elife High-altitude hypoxia exposure inhibits erythrophagocytosis by inducing macrophage ferroptosis in the spleenAuthors - Wan-Ping Yang |
| mouse | IHC | Neuron Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrierAuthors - Yuan Xie |
as a surrogate for the percentageof indels generated within the target genomic region. 7. Analysis of SURVEYOR assays results. To calculate the genomecleavage effi ciency of a tested target, quantify the band intensityof SURVEYOR assay products visualized
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