- ￥1350 - 3000
- Proteintech
- 21017-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KLF5 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
KLF5
- 抗体英文名：
KLF5 Polyclonal antibody
- 抗体名：
KLF5 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15267 Product name: Recombinant human KLF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC042131 Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 5 (intestinal) |
| 别名 | GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2 |
| 计算分子量 | 457 aa, 51 kDa |
| 观测分子量 | 51-55 kDa |
| GenBank蛋白编号 | BC042131 |
| 基因名称 | KLF5 |
| Gene ID (NCBI) | 688 |
| RRID | AB_10696447 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13887 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Commun Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infectionAuthors - Jiakai Hou |
| human | WB | Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.Authors - Shunrong Ji |
| human | WB,IHC | Oncogene KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinomaAuthors - Zhenyu Yang |
| human | WB | Cell Death Dis DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.Authors - Rong-Jie Fu
|
| human | WB,IHC | Aging (Albany NY) Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.Authors - Yilong Li
|
| human | WB | Oncotarget Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.Authors - Xiaolong Wei
|
C. David/赵玉沛院士/吴文铭揭示「癌王发迹史」，为早期诊断与治疗提供潜在的靶点和思路
高表达的转录因子的筛选。研究发现，敲除掉 AP1 家族中的 Fosl1，Junb，Klf 家族中的 Klf5 与 Fox 家族中的 Foxa2，都对胰腺癌的生存产生了阻碍（图三，a）。为了探究 Junb，Fosl1，Klf5 与 Foxa2 在胰腺癌的发展早期的作用，作者利用腺相关病毒血清型（携带 Cdkn2a, Trp53, 和 Smad4 这三个在胰腺癌中高突变的肿瘤抑制基因的 sgRNA 与目标转录因子的 sgRNA）去分别敲除掉这几个转录因子。跟对照组的 PDAC 相比，敲除掉 Junb
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
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