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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human INSR protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IP, ELISA
- 靶点:
INSR/CD220
- 抗体英文名:
INSR/CD220 Polyclonal antibody
- 抗体名:
INSR/CD220 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20433-1-AP targets INSR/CD220 in WB, IHC, IF, IP, CoIP, Dot blot, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP, Dot blot |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish, fish |
| 免疫原 |
CatNo: Ag13922 Product name: Recombinant human INSR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 968-1370 aa of BC117172 Sequence: RKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | INSR |
| 别名 | INSR, CD220, EC:2.7.10.1, HHF5, insulin receptor |
| 计算分子量 | 1382 aa, 156 kDa |
| 观测分子量 | 190 kDa, 135 kDa, 95 kDa |
| GenBank蛋白编号 | BC117172 |
| 基因名称 | INSR |
| Gene ID (NCBI) | 3643 |
| RRID | AB_10646469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06213 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | Dot blot | Environ Health Perspect Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.Authors - Jacob Raber |
| mouse | WB | Int J Biol Macromol Lycium barbarum polysaccharide mitigates high-fat-diet-induced skeletal muscle atrophy by promoting AMPK/PINK1/Parkin-mediated mitophagyAuthors - Yanru Ren |
| rat | WB | Food Funct l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathwaysAuthors - Ling Lin |
| human | WB | Br J Surg MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer.Authors - M Xiao |
| human | WB | FASEB J Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.Authors - Tong Xu |
| mouse | WB,IHC | Sci Rep NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.Authors - Shu Yang |
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
发生,最好在特异性抗体处理切片之前,选择与二抗种属相同的非免疫血清封闭电荷,阻止一抗与之结合,抑制非特异性背景着色。常用方法是用2%~10%羊血清或2%~5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固,所以最好不要冲洗,倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7.第一抗体孵育 滴加特异性一抗于切片上,4℃孵育24~48小时,或室温孵育过夜,也可在37~C孵育1~2小时。之后,用PBS冲洗3次,每次5分钟。一抗分为多克隆抗体和单克
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