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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human PRKCD protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点:
PKC Delta
- 抗体英文名:
PKC Delta Polyclonal antibody
- 抗体名:
PKC Delta Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human brain tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, U-251 cells, HeLa cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19132-1-AP targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5631 Product name: Recombinant human PRKCD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 326-676 aa of BC043350 Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase C, delta |
| 别名 | PRKCD, PKCD, nPKC-delta, MAY1, EC:2.7.11.13 |
| 计算分子量 | 78 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC043350 |
| 基因名称 | PKC Delta |
| Gene ID (NCBI) | 5580 |
| RRID | AB_10637263 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05655 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IHC,IF,IP,CoIP | Oncogene TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCDAuthors - Linqing Sun |
| mouse | WB | Virol Sin Rottlerin plays an antiviral role at early and late steps of Zika virus infectionAuthors - Shili Zhou
|
| rat | WB | Food Chem Toxicol G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.Authors - Yao Liu |
| WB | Fish Shellfish Immunol In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.Authors - Zhong-Wen Chang | |
| mouse | WB | iScience Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli.Authors - Houbao Qi |
| mouse | WB | Neurochem Int Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.Authors - Katarzyna Dąbrowska |
列为5`-TGACTCA-3`)。TRE命名是因为TPA可通过活化PKC而诱导AP-1并与AP-1结合位点结合。 IL-2基因在转录起始部位的5`端含有一个增强子,它仅存在T细胞中以细胞特异性的方式控制IL-2基因的转录,几种结合到IL-2基因增强子区域的DNA结合蛋白被认为是MHC/Ag结合TCR与IL-2基因转录调节之间重要的联系环节。这个增强子中的一些序列可以特异性地同一个称为AP-1的转录激活蛋白相结合。AP-1可存在于不同细胞类型中,许多不同基因的调节区域
用于免疫检测的特殊配方的抗体稀释液,可以促进抗原抗体特异性结合,产生比传统方法高几倍或十几倍的信号强度,从而解决免疫检测实验中(比如免疫印迹,ELISA等)经常遇到的低灵敏度和高背景强度等问题,得到准确清晰的实验结果。 试剂盒操作简单方便,含有溶液1和2两种即用型溶液,分别用做一抗和二抗的稀释液,替代其他的传统稀释缓冲液,包括TBS,TBST,PBS,PBST等。其余操作均按照标准的免疫检测操作流程进行。此外,试剂盒中各组分对标记抗体的酶活性(辣根过氧化物酶HRP,碱性磷酸酶AP等)均没有影响
-AT主要参与T细胞的活化。上面已经提到,胞质中无活性状态的NF-AT以磷酸化的形式存在,称为NF-ATp,钙调磷酸酶作用后使其脱磷酸化而被激活并发生转位。而钙调磷酸酶能发挥作用,是信号转导的结果。 2.NF-κB NF含义同上。κB指B细胞x链,由两个亚单位p50和p65组成,其发现和定名是因为参与B细胞活化。现知它是一种分布广泛和十分重要的转录因子。通常,NF-κB在胞质中和抑制因子LxB以复合物的形式存在。PKC可使I-κB发生磷酸化造成该复合物解离,从而解除了I-κB对NF-κB
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