- ￥1350 - 3000
- Proteintech
- 15833-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human VTN protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Vitronectin/S-protein
- 抗体英文名：
Vitronectin/S-protein Polyclonal antibody
- 抗体名：
Vitronectin/S-protein Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | rat plasma, human plasma, human testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15833-1-AP targets Vitronectin/S-protein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, plasmodium falciparum |
| 免疫原 |
CatNo: Ag8443 Product name: Recombinant human VTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-478 aa of BC005046 Sequence: EVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAMWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vitronectin |
| 别名 | Serum-spreading factor, Somatomedin-B, S-protein, V75, Vitronectin |
| 计算分子量 | 478 aa, 54 kDa |
| 观测分子量 | 70-75 kDa, 65 kDa, |
| GenBank蛋白编号 | BC005046 |
| 基因名称 | VTN |
| Gene ID (NCBI) | 7448 |
| RRID | AB_2216309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
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文献和实验| Species | Application | Title |
|---|---|---|
Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injuryAuthors - Shuyong Wang | ||
| rat | WB,IF | Brain Pathol Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.Authors - Hongjin Wang |
| human | WB,IF | Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.Authors - Takahiro Tougan |
| human | WB,IF | J Cell Mol Med Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.Authors - Mari Kawamura |
| human | Langmuir Characterization of Initial Cell Adhesion on Charged Polymer Substrates in Serum-Containing and Serum-Free Media.Authors - Takashi Hoshiba | |
| human | WB | Proteomics SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.Authors - Hongyu Zhang |
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
.J., Stark, C., Liu, G., Ahn, J., Dewar‐Darch, D., Reguly, T., Tang, X., Almeida, R., Qin, Z.S., Pawson, T., Gingras, A.C., Nesvizhskii, A.I., and Tyers, M. 2010. A global protein kinase and phosphatase interaction network in yeast. Science 328:1043
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
600, then dilute the cells to final OD600 of 0.5, place on ice. Screening Plaques with AP-Fusion Protein Reagents: 1M IPTG stock solution NZY plates with phage 20 x
, A. & Hosseinkhani, S. Split-luciferase complementary assay:applications, recent developments, and future perspectives. Anal Bioanal Chem 406, 5541-5560, doi:10.1007/s00216-014-7980-8 (2014).[5]. Nickerson,A., Huang, T., Lin, L. J. & Nan, X. Photoactivated
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