- ￥1350 - 3000
- Proteintech
- 15303-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GBP1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
GBP1
- 抗体英文名：
GBP1 Polyclonal antibody
- 抗体名：
GBP1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human placenta tissue, human brain tissue, HUVEC cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15303-1-AP targets GBP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
CatNo: Ag7562 Product name: Recombinant human GBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-592 aa of BC002666 Sequence: VHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GTP binding protein 1 |
| 别名 | Guanylate-binding protein 1, Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC002666 |
| 基因名称 | GBP1 |
| Gene ID (NCBI) | 2633 |
| RRID | AB_2247448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32455 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.
What is the molecular weight of GBP1?
The molecular weight of GBP1 is 68kDa.
What is the cellular localization of GBP1?
GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).
What is the role of GBP1 in immunity?
GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.
What is the role of GBP-1 in colorectal carcinoma?
In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Nat Immunol Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.Authors - Michal P Wandel
|
| human | WB | Nat Cell Biol LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.Authors - Gaopeng Li |
| human | WB | Cell Host Microbe GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8.Authors - Michal P Wandel |
| human | WB,IP | J Med Virol Guanylate-binding protein 1 inhibits Hantaan virus infection by restricting virus entryAuthors - Tianle Gu |
| human | WB | Nat Commun Mechanistic insight into bacterial entrapment by septin cage reconstitution.Authors - Damián Lobato-Márquez |
| human | WB,IHC,IF | J Transl Med Function and mechanism of GBP1 in the development and progression of cervical cancerAuthors - Senyu Wang
|
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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