- ￥1350 - 3000
- Proteintech
- 15625-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human FCGR2A protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
FCGR2A / CD32a
- 抗体英文名：
FCGR2A / CD32a Polyclonal antibody
- 抗体名：
FCGR2A / CD32a Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | K-562 cells, U-937 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15625-1-AP targets FCGR2A / CD32a in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8072 Product name: Recombinant human FCGR2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-316 aa of BC020823 Sequence: MTMETQMSQNVCPRNLWLLQPLTVLLLLASADSQAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGVIVAVVIATAVAAIVAAVVALIYCRKKRISANSTDPVKAAQFEPPGRQMIAIRKRQLEETNNDYETADGGYMTLNPRAPTDDDKNIYLTLPPNDHVNSNN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Fc fragment of IgG, low affinity IIa, receptor (CD32) |
| 别名 | CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC020823 |
| 基因名称 | CD32a |
| Gene ID (NCBI) | 2212 |
| RRID | AB_2246912 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,rat | WB | Int J Mol Sci Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD PathwayAuthors - Pan Lei |
| rat | WB | Exp Neurol Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.Authors - Luodan Yang |
| human | WB | Am J Pathol FcγRIIa ligation induces platelet hypersensitivity to thrombotic stimuli.Authors - Berlacher Mark D MD |
| rat | WB | J Alzheimers Dis Treadmill Exercise Exerts Neuroprotection and Regulates Microglial Polarization and Oxidative Stress in a Streptozotocin-Induced Rat Model of Sporadic Alzheimer's Disease.Authors - Yujiao Lu |
| mouse | WB | Cell Death Dis Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32.Authors - Jianbin Yin |
| mouse | WB,IF | Int Immunopharmacol Grossamide attenuates inflammation by balancing macrophage polarization through metabolic reprogramming of macrophages in miceAuthors - Wenlong Zhao |
10X TBS：24.2 g Trisbase, 80 g NaCl；用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T)：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体
10X TBS：24.2 g Trisbase, 80 g NaCl;用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T)：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体
7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液（TBS/T）：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体，脱脂奶粉用于单克隆抗体，这样可得到较高的信噪比。预染的蛋白质Marker，可用于监测转膜的效率。 样品制备 原始样品可为细胞、组织
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