- ￥1350 - 3000
- Proteintech
- 15073-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IP, ELISA
- Rabbit
- human, mouse, rat, monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human METTL3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, monkey
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IP, ELISA
- 靶点：
METTL3
- 抗体英文名：
METTL3 Polyclonal antibody
- 抗体名：
METTL3 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HT-1080 cells, mouse testis tissue, HepG2 cells, Jurkat cells, Neuro-2a cells, A549 cells, NCCIT cells, COS-7 cells, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15073-1-AP targets METTL3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, drosophila |
| 免疫原 |
CatNo: Ag7110 Product name: Recombinant human METTL3 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 229-580 aa of BC001650 Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 3 |
| 别名 | EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001650 |
| 基因名称 | METTL3 |
| Gene ID (NCBI) | 56339 |
| RRID | AB_2142033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86U44 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nature METTL3 regulates heterochromatin in mouse embryonic stem cells.Authors - Wenqi Xu
|
| mouse | WB | Nature The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.Authors - Jiadong Liu
|
| human | WB | Nature mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.Authors - Junho Choe
|
| human | WB | Nature N(6)-methyladenosine-dependent regulation of messenger RNA stability.Authors - Wang Xiao X
|
| human | WB | Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasisAuthors - Kaixuan Zeng
|
| mouse | WB,IF | Science Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.Authors - Shay Geula
|
Stem Cell Rep：高绍荣团队揭示 Mettl14 以不依赖于 m⁶A 的方式驱动衰老相关分泌表型促进体细胞重编程
N6-甲基腺苷（m6A）修饰与人类疾病息息相关，它通过影响包括细胞周期、细胞命运决定和细胞稳态等 1 生命进程的多个方面来调节人类疾病。m6A 修饰的建立由 Mettl3-Mettl14 甲基转移酶复合物（MTC）催化 2。通过 Yamanaka 因子（Oct4、Sox2、Klf4 和 c-Myc，称为 OSKM）将体细胞重编程为诱导性多能干细胞（iPSCs）3，为研究细胞命运转变的分子机制提供了一个很好的研究系统。之前研究表明 Mettl3 的过量达可以增加 m6A 的水平，促进体细胞重编
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
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