- ￥1350 - 3000
- Proteintech
- 15017-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RABL4 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
IFT27
- 抗体英文名：
IFT27 Polyclonal antibody
- 抗体名：
IFT27 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human RPE cells |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15017-1-AP targets IFT27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6967 Product name: Recombinant human RABL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC000566 Sequence: MVKLAAKCILAGDPAVGKTALAQIFRSDGAHFQKSYTLTTGMDLVVKTVPVPDTGDSVELFIFDSAGKELFSEMLDKLWESPNVLCLVYDVTNEESFNNCSKWLEKARSQAPGISLPGVLVGNKTDLAGRRAVDSAEARAWALGQGLECFETSVKEMENFEAPFHCLAKQFHQLYREKVEVFRALA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB, member of RAS oncogene family-like 4 |
| 别名 | RABL4, Rab-like protein 4, Rab like protein 4, Putative GTP-binding protein RAY-like, BBS19 |
| 计算分子量 | 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC000566 |
| 基因名称 | RABL4/IFT27 |
| Gene ID (NCBI) | 11020 |
| RRID | AB_2878100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW83 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Cell Biol Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.Authors - Malavika Raman
|
| human | WB | Mol Cell Proteomics CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.Authors - Tina Beyer |
| human | WB | Anal Chem Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer CellsAuthors - Zi Gao |
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
抗原修复方法 Antigen Retrieval Methods
虽然固定对于保持组织形态至关重要，但该过程也可能对免疫组化/免疫细胞化学检测产生负面影响。固定会改变蛋白质的生化性质，导致目标表位被掩盖，从而无法与一抗结合。表位被掩盖的原因可能是表位内氨基酸的交联、表位附近或位点无关肽段的交联、表位构象的改变或抗原静电荷的改变。抗原修复是指任何能够逆转表位掩盖并恢复表位-抗体结合的技术。 抗原修复技术 抗原修复的必要性取决于多种因素，包括但不限于目标抗原、所用抗体、组织类型以及固定方法和时间。由于多克隆抗体能够识别多个表位，因此与单克隆抗体相比，它们无需抗原
RUBIS-0021 RUBIS瑞比士 镊子41LB-5 41LB-5 RUBIS-0020 RUBIS瑞比士 镊子PP PP RUBIS-0019 RUBIS瑞比士 镊子27 27 RUBIS-0018 RUBIS瑞比士 镊子C44 C44 RUBIS-0017 RUBIS瑞比士 镊子15AP 15AP RUBIS-0016 RUBIS瑞比士 镊子43LB-8PEEK 43LB-8PEEK RUBIS-0015 RUBIS瑞比士
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