- ￥1350 - 3000
- Proteintech
- 14075-1-AP
- 2025年10月13日
- IHC, IF/ICC, IF-P, ELISA
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PDGFD protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, IF/ICC, IF-P, ELISA
- 靶点：
PDGF-D
- 抗体英文名：
PDGF-D Polyclonal antibody
- 抗体名：
PDGF-D Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14075-1-AP targets PDGF-D in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | IHC, IF/ICC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 |
CatNo: Ag5154 Product name: Recombinant human PDGFD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-364 aa of BC030645 Sequence: MHRLIFVYTLICANFCSCRDTSATPQSASIKALRNANLRRDDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | platelet derived growth factor D |
| 别名 | PDGFD, Platelet-derived growth factor D, latent form, Platelet-derived growth factor D, PDGFD receptor binding form, PDGF D |
| 计算分子量 | 43 kDa |
| GenBank蛋白编号 | BC030645 |
| 基因名称 | PDGFD |
| Gene ID (NCBI) | 80310 |
| RRID | AB_2162208 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Cell Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor.Authors - Alexander D Barrow |
| human | IHC,IF | Cell Oncol (Dordr) Exploring the impact of PDGFD in osteosarcoma metastasis through single-cell sequencing analysisAuthors - Yujing Huang |
| human | WB | Cell Physiol Biochem Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.Authors - Qin Zheng |
| human | IHC | J Immunol Cutting Edge: PDGF-DD Binding to NKp44 Costimulates TLR9 Signaling and Proinflammatory Cytokine Secretion in Human Plasmacytoid Dendritic CellsAuthors - Alexander David Barrow |
| mouse | WB | Front Pharmacol M2 Macrophages Promote PDGFRβ+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.Authors - Ziyu Li |
| rat | IHC | ACS Omega Promoted Skin Wound Healing by Tail-Amputated Eisenia foetida Proteins via the Ras/Raf/MEK/ERK Signaling PathwayAuthors - Tianyi Li |
分为磷酸化一抗、非磷酸化一抗两大类。每一大类又包含若干，不知如何选择，它们之间有何区别？实验目的为研究某细胞AP-1的表达情况，及其蛋白磷酸化和DNA结合活性等。 表达 和 结合 ２个方面都考察? 目的蛋白是ＡＰ-１中的c-jun一个? 不考察c-fos之类的? 还有, 结合可是无法用ＷＢ考察的:D:D chuckdouble ziyunfei wrote:
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
coupled with mass spectrometry (AP?MS) is a robust technique used to identify protein?protein interactions. With recent improvements in sample preparation, and dramatic advances in MS instrumentation speed and sensitivity, this technique
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