- ￥1350 - 3000
- Proteintech
- 13032-1-AP
- 2025年10月13日
- WB, IHC, IF-P, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ELAVL4 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IP, ELISA
- 靶点：
HuA/B/C/D
- 抗体英文名：
HuA/B/C/D Polyclonal antibody
- 抗体名：
HuA/B/C/D Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human cerebellum tissue, mouse cerebellum tissue, PC-13 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13032-1-AP targets HuA/B/C/D in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3678 Product name: Recombinant human ELAVL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC036071 Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVGQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAITSLNGYRLGDRVLQVSFKTNKAHKS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| 别名 | HUD, ELAVL4, ELAV like protein 4, Hu-antigen D, Hu C/D |
| 计算分子量 | 380 aa, 42 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC036071 |
| 基因名称 | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2096385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26378 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | IHC | J Neurosci Methods Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF.Authors - Ruth Butler-Ryan |
| human | IHC,WB | J Cosmet Dermatol Study on the correlation of high expression level of HuD with seborrheic keratosis and skin agingAuthors - Juping Chen |
| mouse | IF | Med Mol Morphol Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T miceAuthors - Nami O Yamada |
| mouse | IF | Biomed Pharmacother Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axisAuthors - Jinzhao Bai |
and 10X PCR buffer. PCR program: 95¡C X 3'; 30 X (95¡C X 1', 55¡C X 1', 72¡C X 3');72¡C X 3' PCR for FGF9 KO mice Oligos... 5’ oligo: (JC46): 5’-GCA AGG GAG GGG AGT TGG ATA TAC C-3’ 3’ oligo: (JC42): 5’-CAG CCC AAG CTT TCG CGA GCT CG
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
address. (C ) Standard Web browser on the host computer (here, Mozilla Firefox on Mac OS X). ProHits Lite VM may be accessed by typing the IP address from panel B into the host computer browser. Other computers on the network can access the VM
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
on ice). Replace blocking solution with incubation solution. Incubate overnight at 4°C with gentle shaking. Wash membranes for 4 x 15 minutes with PBS + 0.1% Triton X-100 at room temperature on shaker. Rinse once with AP buffer
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