- ￥1350 - 3000
- Proteintech
- 12922-3-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HDAC2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
HDAC2
- 抗体英文名：
HDAC2 Polyclonal antibody
- 抗体名：
HDAC2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12922-3-AP targets HDAC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3607 Product name: Recombinant human HDAC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 274-571 aa of BC031055 Sequence: TDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTHETAVALDCEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone deacetylase 2 |
| 别名 | EC:3.5.1.-, EC:3.5.1.98, HD2, histone deacetylase 2, RPD3 |
| 计算分子量 | 458 aa, 52 kDa; 488 aa,55 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC031055 |
| 基因名称 | HDAC2 |
| Gene ID (NCBI) | 3066 |
| RRID | AB_2118516 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| rat | IP | Cell Metab Sphingolipid metabolism controls mammalian heart regenerationAuthors - Xiaoqian Ji |
| human | CoIP | J Clin Invest SAP30 promotes breast tumor progression by bridging the transcriptional corepressor SIN3 complex and MLL1Authors - Lei Bao |
| human | WB | Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.Authors - Xin Sun |
| mouse,human | IHC,IF | Acta Pharm Sin B PIM1-HDAC2 axis modulates intestinal homeostasis through epigenetic modificationAuthors - Jianming Yang |
| mouse | WB | J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.Authors - Shanshan Zhong |
| mouse | IP | Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.Authors - Xinzhi Li |
技术资料暂无技术资料 索取技术资料
