- ￥1350 - 3000
- Proteintech
- 11227-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ACTG1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
Gamma Actin
- 抗体英文名：
Gamma Actin Polyclonal antibody
- 抗体名：
Gamma Actin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human colon tissue, human skin cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11227-1-AP targets Gamma Actin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1728 Product name: Recombinant human ACTG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC009848 Sequence: MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, gamma 1 |
| 别名 | ACTG1, Actin, cytoplasmic 2, N-terminally processed, Actin, cytoplasmic 2, actin, actg 1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC009848 |
| 基因名称 | Gamma Actin |
| Gene ID (NCBI) | 71 |
| RRID | AB_2223507 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63261 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | iScience A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heartAuthors - Aude Angelini |
| mouse | WB | Front Mol Neurosci F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.Authors - Xiao-Zhou Liu |
| human | WB | Front Neurosci Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic ratsAuthors - Shiquan Cui |
| mouse | WB | FEBS J Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathwayAuthors - Ju Liu |
| human | WB | Ultrasound Q Ultrasound Microbubble-Stimulated miR-145-5p Inhibits Malignant Behaviors of Breast Cancer Cells by Targeting ACTG1Authors - Liang Ren |
| human | WB,IF | Cells Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and ActinAuthors - Ai Fujimoto |
实验原理 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术，这是一种能够判断血液中三种免疫球蛋白IgM，IgG，IgA含量水平的实验方法。在这项实验技术中，首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离，然后将特异性的抗体引入到与电泳方向平行的凹槽中，在抗原和抗体的扩散过程中，抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用于洗去未结合的蛋白，抗原和抗体结合所形成
Functional Characterization of Proteins Regulating Actin Assembly
. Combeau, C. and Carlier, M.F. 1989. Characterization of the aluminum and beryllium fluoride species bound to F‐actin and microtubules at the site of the gamma‐phosphate of the nucleotide. J. Biol. Chem. 264
相关专题 【实验原理】 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术，这是一种能够判断血液中三种免疫球蛋白IgM, IgG, IgA含量水平的实验方法。在这项实验技术中，首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离，然后将特异性的抗体引入到与电泳方向平行的凹槽中，在抗原 和抗体的扩散过程中，抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用
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