- ￥1350 - 3000
- Proteintech
- 10827-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KAT5 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
TIP60/KAT5
- 抗体英文名：
TIP60/KAT5 Polyclonal antibody
- 抗体名：
TIP60/KAT5 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10827-1-AP targets TIP60/KAT5 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1261 Product name: Recombinant human KAT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-457 aa of BC000166 Sequence: ATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 5 |
| 别名 | 60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 58 kDa, 70 kDa |
| GenBank蛋白编号 | BC000166 |
| 基因名称 | KAT5 |
| Gene ID (NCBI) | 10524 |
| RRID | AB_2128431 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92993 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature ANP32E is a histone chaperone that removes H2A.Z from chromatin.Authors - Arnaud Obri |
| mouse | WB | Nat Immunol The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells.Authors - Jing Geng |
Acta Pharm Sin B Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy.Authors - Yancheng Tang | ||
Mol Cell PMID: 28366643Authors - Sophie Barral | ||
| human | WB | Nat Commun Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.Authors - Zhiyuan You
|
| human | WB | Cancer Lett Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progressionAuthors - Jiazhou Liu |
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
Dechorionate directly on apple juice/agar plates with 50-60 chlorox; embryos will float to the surface after 2-3''. Collect embryos on a Millipore device with a nitex filter; rinse well with 0.1 Triton X-100 from a squirt bottle
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
conjugate in PBT, rotate 30'. Wash 3X in PBT with a final wash in AP buffer if using AP-conjugate.. The AP staining conditions are as described in the BRL handout that comes with the conjugate. Although the AP stain is more sensitive than HRP
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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