- ￥1350 - 3000
- Proteintech
- 10450-1-AP
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cx32 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
Connexin 32
- 抗体英文名：
Connexin 32 Polyclonal antibody
- 抗体名：
Connexin 32 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, A375 cells, human liver tissue, mouse skin tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10450-1-AP targets Connexin 32 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0659 Product name: Recombinant human Cx32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-283 aa of BC002805 Sequence: TYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gap junction protein, beta 1, 32kDa |
| 别名 | Connexin-32, GJB1, CMTX, CMTX1, CX32 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC002805 |
| 基因名称 | Connexin-32 |
| Gene ID (NCBI) | 2705 |
| RRID | AB_2110629 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08034 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connexin 32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin 32 is the major gap junction protein of liver, and the expression is also found in the brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin 32 plays an important role in peripheral nerve. Defects in Connexin 32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. Connexin 32 protein can be detected as a 27-32 kDa monomer and a 48-54 kDa dimer (PMID: 8266101; 8613761; 8608591; 19265674; 17972320; 19845480)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Chemosphere Organophosphate flame retardant TDCPP: A risk factor for renal cancer?Authors - Xuan Zhou |
| human | WB | World J Gastroenterol PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.Authors - Xiao-Ming Liu |
| mouse | WB | Brain Res Bull Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.Authors - Chen Men |
| human | IHC | J Biomed Biotechnol Ischemia alters the expression of connexins in the aged human brain.Authors - Nakase Taizen T |
| human | IHC,IF | Histopathology Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.Authors - Wen-Qun Zhong |
| human | WB | Oncol Lett Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.Authors - Lihua Huang |
Regulation and Control of AP-1 Binding Activity in Embryotoxicity
is generally detected with autoradiography of the 32 P-labeled DNA. The underlying principle is that nucleic acid with protein bound to it will migrate more slowly through a gel matrix than the free nucleic acid. In this chapter, a representative protocol
一、批量提取法 称取DEAE-纤维素(DE32或DE52)50g，置于1000ml烧杯中，先以蒸馏水漂浮除去细颗粒，再经酸碱处理后，用0.01～0.05mol/L，pH8.0左右的磷酸盐缓冲液(PB)平衡。将水分抽干，或用布氏滤斗(内放两层滤纸)过滤，收集湿纤维素，以降低其离子强度。按1ml血清加湿重5g DEAE纤维素，经过充分搅拌，置4℃吸附1h。上清液可再如此处理一次，即获得较纯的IgG。该法提取IgG简便，既可小量提取，也可大量制备。 二、Tris-Cl
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
of the software to analyze AP?MS data. Curr. Protoc. Bioinform. 39:8.16.1?8.16.32. © 2012 by John Wiley & Sons, Inc. Keywords: affinity purification coupled with mass spectrometry; data analysis; virtual machine; statistical models; protein
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