生物素抗体,Agarose亲和填料

关于 Biotin Antibody Agarose 的详细介绍

Product Description	The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins.
Formulation	1 mL beads in 2 mL slurry with glycerol
Antibody Immobilized	10 mg of biotin antibody/mL agarose
Binding       Capacity	> 1 mg/mL of biotinylated IgY
Applications	IP; Immuno-affinity chromatography; 10 mg/mL for proteomic study
Scientific Description	Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding could be competitively eluted with free biotin.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Do not aliquot and shake throughly before use. Expiration date is one year from date of shipping if properly stored.

 

蛋白质翻译后生物素化修饰是新的PTM研究领域，Nature刊登的科技文献指出： 通过比较Sigma,SantaCruz的同类产品，只有ImmuneChem的抗生物素抗体Agarose填料(货号：ICP0615）达到PTM蛋白质组学质谱分析应用的质量。

 

抗生物素抗体Agarose填料(货号：ICP0615）也是非破坏性（Non-invasive)纯化Biotin-tag重组蛋白的重要填料,可不必用蛋白变性溶液而直接用游离生物素洗脱。 比传统的Avidin， Streptavidin Agarose要更理想，更可靠！

 

根据哈佛大学发表的文章数据显示，用我们的产品进行抗生物素蛋白富集和质谱分析，在数百种蛋白质上产生了1,600个以上的生物素化位点，所能检测到的生物素化位点比Sigma,SantaCruz的同类产品能多30倍！
文章链接：https://www.nature.com/articles/nmeth.4465#auth-1

ImmuneChem Biotin Antibody Agarose produced the best results in research article  published in: Nature Methods  Volume 14, Issue 12, 16 October 2017, Pages 1167–1170
The binding affinity of  anti-biotin antibodies could improve the elution of biotinylated peptides.  This table shows the difference in the # of unique peptides when samples are  biotinylated.	Biotin antibody compared  to non-biotin samples
Immunechem’s Biotin Antibody  Agarose (ICP0615) yielded the highest number of biotinylated peptides compared  to Sigma-Aldrich and Santa Cruz. For  more details, please refer to:  Nature  Methods Volume  14, Issue 12, 16 October 2017, Pages 1167–1170

Biotinylated BSA	ICP0614	5mg/支		2700元
Biotin Antibody, Rabbit	ICP0611	1mg/支	ELISA WB IHC IP IC	2600元
Biotin Antibody, HRP	ICP0612	1mg/支	ELISA WB	2950元
Biotin Antibody, CY5.5	ICP0698	100ug/支		1950元
Biotin Antibody Agarose	ICP0615	10mg/1ml	IAC, IP	6950元

ImmuneChem（中国）

南宁市奥思特科技有限责任公司   萧廉明

地址：南宁市高新区科园大道68号东盟慧谷11栋203-205室

电话：18775373503  QQ：2602622833

公司网站：http://www.ost-biotech.com/

技术支持：（Hao Xiao博士）immunechem@yahoo.com

 

我们可以为您提供下列产品和服务：

1：修饰蛋白/多肽蛋白质组学研究所用的抗体及亲和填料：

  蛋白质甲基化、乙酰化、磷酸化， 生物素化，赖氨酸羧甲基化（老化糖化蛋白），半胱氨酸化，及半胱氨酸羧甲基化 等翻译后修饰蛋白质组学的泛特异性抗体及其用于纯化富集的抗体Agarose亲和填料！

 

2：修饰蛋白/多肽蛋白质组学研究服务：

  提供蛋白质修饰多肽位点的蛋白质组学测序服务，修饰蛋白/多肽差异 表达的质谱分析。申请生理病理状态下修饰的蛋白研究的课题提供构想！