The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins.
Formulation
1 mL beads in 2 mL slurry with glycerol
Antibody Immobilized
10 mg of biotin antibody/mL agarose
Binding Capacity
> 1 mg/mL of biotinylated IgY
Applications
IP; Immuno-affinity chromatography; 10 mg/mL for proteomic study
Scientific Description
Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding could be competitively eluted with free biotin.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Do not aliquot and shake throughly before use. Expiration date is one year from date of shipping if properly stored.
The binding affinity of anti-biotin antibodies could improve the elution of biotinylated peptides. This table shows the difference in the # of unique peptides when samples are biotinylated.
Biotin antibody compared to non-biotin samples
Immunechem’s Biotin Antibody Agarose (ICP0615) yielded the highest number of biotinylated peptides
compared to Sigma-Aldrich and Santa Cruz.
For more details, please refer to: Nature Methods
Volume 14, Issue 12, 16 October 2017, Pages 1167–1170