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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Shanghai Model Organisms Center, Inc.
- 库存:
1
- 供应商:
上海南方模式生物科技股份有限公司
- 规格:
品系
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文献和实验Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
both in vitro and in vivo to produce a nuclear staining pattern. Protocol Tissue and cells are fixed with 4% paraformaldehyde in vitro for 30 mins at 4ºC, in vivo post fix for two hours at room temperature (r.t.) Following fixation, wash in 0.1M Phosphate
Sulphate 430 550 Bisbenzamide 360 600-610 BOBO-1, BO-PRO-1 462 481 Blancophor FFG Solution 390 470 Blancophor SV 370 435 Bodipy Fl 503 512 Bodipy TMR 542 574 Bodipy TR 589 617 BOPRO 1 462 481 Brilliant Sulpho-flavin FF 430 520 Calcein 494 517
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