单位定义:一个单位的SuperScript® III RT 是在37°C 的反应体系中,使用poly(A)•oligo(dT)12-18 作为模板•引物,在10 分钟内将1 纳摩尔脱氧核糖核苷酸掺入酸沉淀材料所需的酶量(3)。
单位反应条件:50 mM Tris-HCl (pH 8.3)、40 mM KCl、6 mM MgCl 2、1 mM DTT。0.5 更看更多mM [ 3 H]dTTP、0.1 mM poly(A)、0.1 mM oligo(dT)12-18、0.1 mg/ml BSA 和酶,共50 µl,温度37°C,时间10 分钟。
详细说明
常用规格
Optimal Reaction Temperature:
50° C
Final Product Size(s):
12.3 kb or less
Ribonuclease H Activity:
Reduced
Concentration:
200 units/µl
Form:
Frozen
Format:
Tube(s)
Template:
ssRNA
Sensitivity:
Medium
Product Size:
10,000 units
Enzyme Function:
RNA dependent DNA Polymerase
Number of Reactions:
50 Reactions
Regulatory Statement:
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
内容和储存
SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT. Store at -20°C. Guaranteed stable for 6 months when properly stored.