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上海觅拓
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文献和实验The thiobarbituric acid (TBA) assay was developed to quantitatively determine lipid peroxidation for aldehydic compounds in biological matrices. Kohn and Liversedge introduced this methodology in 1944 ( 1 , 2 ). Since its introduction, the TBA
Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity
polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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