- ￥960 - 1600
- Solarbio
- 北京
- K000013M
- 2025年07月16日
- IF ; IHC ; ICC ; WB
- Mouse
- Rat ; Mouse ; Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant human HP1 alpha protein fragments expressed in E.coli.
- 亚型：
IgG2b
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 标记物：
详询
- 适应物种：
Rat ; Mouse ; Human
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
K000013M
- 级别：
详询
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
IF ; IHC ; ICC ; WB
- 浓度：
详询
- 靶点：
HP1-α
- 抗体英文名：
Anti-HP1-α Monoclonal Antibody
- 抗体名：
HEL25;HP1;HP1A
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
|
英文名称
|
Anti-CBX5 Monoclonal Antibody
|
|
靶标
|
CBX5
|
|
别名
|
Antigen p25, HP1-α, CBX5, CBX5_HUMAN, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox homolog 5, Chromobox protein homolog 5, Heterochromatin protein 1 alpha, Heterochromatin protein 1, Heterochromatin protein 1 homolog alpha, HP1 alpha, HP1 alpha homolog, HP1, HP1A, HP1Hs alpha
|
|
背景资料
|
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
|
|
Swiss Prot
|
P45973
|
|
Gene ID
|
23468
|
|
亚细胞定位
|
Chromosome Nucleus centromere
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG2b
|
|
克隆号
|
3G2-H10-A6
|
|
交叉反应
|
Rat ; Mouse ; Human
|
|
蛋白分子量
|
22kDa
|
|
应用
|
IF ; IHC ; ICC ; WB
|
|
稀释比例
|
WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ICC 1:50-200.
|
|
免疫原
|
Recombinant protein of human CBX5
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
