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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year
- 保质期:
Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year
- 英文名:
MG-132
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- CAS号:
133407-82-6
- 规格:
5mg/10mg/25mg/10mM*1mL in DMSO/50mg/100mg
| 规格: | 5mg | 产品价格: | ¥320.00 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥430.00 |
| 规格: | 25mg | 产品价格: | ¥920.00 |
| 规格: | 10mM*1mL in DMSO | 产品价格: | ¥360.00 |
| 规格: | 50mg | 产品价格: | ¥1360.00 |
| 规格: | 100mg | 产品价格: | ¥2140.00 |
MG-132是一种新颖,特异性,有效,可逆和可渗透细胞的蛋白酶体抑制剂,并且还抑制钙蛋白酶.还是一种多肽醛,一种有效的自噬 (autophagy) 激活剂。
In Vitro
Cell(泛素化实验,MDA-MB-231 and MDA-MB-468 cells,10µg/mL 12 h)
MDA-MB-231 and MDA-MB-468 cells were treated with 10µg/mL MG132, respectively, (Solarbio, IM0310) for 12 h. Cell lysates were obtained using PierceTM IP lysis buffer supplemented with a cocktail and then incubated with anti-MEKK2 antibody and Protein A/G beads overnight at 4℃. Finally, protein A/G beads with the bound immunoprecipitates were collected and analyzed using Western blotting.
来源文献:Song R, Guo P, Ren X, Zhou L, Li P, Rahman NA, Wołczyński S, Li X, Zhang Y, Liu M, Liu J, Li X. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer. Mol Cancer. 2023 Jul 5;22(1):104. doi: 10.1186/s12943-023-01806-x. PMID: 37408008; PMCID: PMC10320902.


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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的:观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法:在细胞培养的不同时间,用α-磷酸奈酚法测定碱性磷酸酶(ALP)活性;放射免疫法测定骨钙素(BGP)含量;半定量逆转录聚合酶链反应(RT-PCR)测I型胶原、MMP-1、TIMP-1基因mRNA表达;Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果:人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
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