Recombinant Allergen Ara h 3.0

Glycinin, Arah3.

Glycinin Ara h 3 is a seed storage protein, 11 S globulin and trypsin inhibitor from peanut. Each subunit of the hexamer is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond. Ara h 3 and Ara h 4 are isoforms. Glycinin is the source of sulfur-containing amino acids in seed meals and it exists in the seeds of many leguminous and non-leguminous plants.

Recombinant Glycinin produced in E. coli is a non- glycosylated, polypeptide chain having a calculated molecular mass of 63 kDa.

Glycinin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered clear solution.

Glycinin is supplied in 20mM HEPES buffer pH-8, 6M Urea and 0.25M NaCl.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Greater than 95% as determined by SDS-PAGE.

1. Binds IgE type human antibodies.
2. Immunodot test with positive/negative sera panels.

SDS

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