Recombinant Human R-Spondin-3

R-spondin-3, Protein with TSP type-1 repeat, hPWTSR, Roof plate-specific spondin-3, hRspo3, Thrombospondin type-1 domain-containing protein 2, RSPO3, PWTSR, THSD2, THSD2, CRISTIN1.

R-spondin-3 (RSPO3) belongs to the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein which is widely expressed in many tissues. RSPO3 contains 2 Furin-like repeats which have been found in various eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain. RSPO3 acts as an activator of the beta-catenin signaling cascade, initiating TCF-dependent gene activation.

Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid Gln at position 22 to amino acid Val at position 201, fused to an FC, 6 x His-tag at C-terminus, containing a total of 498 amino acids and having a Mw of 47.9 kDa. The protein migrates at 61kDa on SDS-PAGE. 
RSPO3 is a truncated protein that lacks amino acid Gln at position 201 to amino acid H at position 272 and purified by proprietary chromatographic techniques.

HEK293 cells.

Sterile Filtered White lyophilized (freeze-dried) powder.

RSPO3 was lyophilized from a 0.2µm filtered solution in 20mM PB, and 150mM NaCl pH-7.2.

It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Greater than 95% as determined by SDS PAGE.

SDS

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