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- pro-366
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
NMM
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/1mg
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥73000.0 |
CATALOGUE NUMBER
PRO-366
SYNONYMS
INTRODUCTION
DESCRIPTION
The NMM is purified by proprietary chromatographic techniques.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
NMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.
STABILITY
Please prevent freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by SDS-PAGE.
BIOLOGICAL ACTIVITY
Assayed for phosphorilation by MLCK.
AMINO ACID SEQUENCE
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Non-Muscle Myosin-II Regulatory Light Chain Human Recombinant full length expressed in E.coli.
The NMM is purified by proprietary chromatographic techniques.
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
【求助】请教：myosin 2 和 nonmuscle myosin2有何区别
between the head and tail. These light chains are often referred to as the essential light chain and the regulatory light chain. In muscle cells, it is myosin II that is responsible for producing the contractile force. Here, the long coiled-coil tails
技术资料