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Recombinant Human Thyroid Pero

xidase
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  • ¥1080 - 87906
  • Prospecbio
  • 以色列
  • enz-285
  • 2026年01月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      TPO

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/1mg

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:1mg产品价格:¥87906.0

    产品细节图片1

    CATALOGUE NUMBER

    ENZ-285

    SYNONYMS

    Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.

    INTRODUCTION

    Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the API-CAl plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.

    DESCRIPTION

    Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.
    The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

    SOURCE

    Sf9 insect cells.

    FORMULATION

    TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.

    COATING CONCENTRATION

    0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

    PURITY

    Greater than 95% as determined by Densitometric Analysis

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. 
    Store, frozen at -20°C for longer periods of time. 
    Avoid multiple freeze-thaw cycles.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.
    The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

    相关实验
    • Human Thyroid Culture

      The technique described in this chapter enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. Human thyroid cells

    • Human Thyroid Epithelial Cells

      The thyroid gland contains two populations of epithelial cells, of quite different embryological origin and function. Only the major component—the follicular cells—will be considered here. The minor C cell population, which forms

    • Transfection and Transformation of Human Thyroid Epithelial Cells

      cells, but again is applicable only to cloned genes of restricted size, and safety considerations are likely to restrict greatly the use of retroviral vectors suitable for human cells. Calcium phosphate coprecipitation has been used with success

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    文献支持
    Recombinant Human Thyroid Peroxidase
    ¥1080 - 87906