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- enz-308
- 2026年01月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
TaqDNA
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
1,000U/3,000U/10,000U
| 规格： | 1,000U | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 3,000U | 产品价格： | ￥2415.0 |
| 规格： | 10,000U | 产品价格： | ￥5800.0 |
CATALOGUE NUMBER
ENZ-308
SYNONYMS
DESCRIPTION
SOURCE
FORMULATION
UNIT DEFINITION
10X REACTION BUFFER WITH MGCL2
10X REACTION BUFFER WITHOUT MGCL2
SPECIFY YOUR OWN REACTION CONDITIONS
STABILITY
Stable for 5 days at 10°C, for longer period of time store at -20°C.
STORAGE BUFFER
50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.
PURITY
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
out the E.coli strain carrying the Taq polymerase gene on an LB amp plate and innoculate a 5 ml overnight culture with a single colony.• Innoculate a 1 liter culture of LB-amp with the 5 ml of overnight culture and grow to an A600 of 0.2.Add IPTG to a final
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl
Dideoxy Sequencing Reactions Using Taq Polymerase
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus . Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain
技术资料