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- Prospecbio
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- enz-309
- 2026年01月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
Taq Plus DNA
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
250U/750U/2,500U
| 规格： | 250U | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 750U | 产品价格： | ￥2415.0 |
| 规格： | 2,500U | 产品价格： | ￥5800.0 |
CATALOGUE NUMBER
ENZ-309
SYNONYMS
DESCRIPTION
SOURCE
UNIT DEFINITION
STORAGE BUFFER
FORMULATION
10X REACTION BUFFER
100 mM KCI
100 mM (NH4)2SO4
20 mM Mg SO4
1% Triton X-100
1 mg/ml bovine serum albumin (BSA).
NOTE
REACTION CONDITIONS
OPTIMIZATION OF DNA SYNTHESIS
2. 10 x reaction buffer.
3. dNTPs.
4. DNA template and primers.
5. Taq Plus.
STABILITY
Stable for 5 days at 10°C, for longer period of time store at -20°C.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl
Dideoxy Sequencing Reactions Using Taq Polymerase
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus . Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain
Cloning of Taq polymerase-amplified PCR products
2) Check the PCR product by agarose gel electrophoresis. You should see a single, discrete band. If you do not see a single band, refer to the Note below. 2. Optional Protocol with Platinum® Taq DNA Polymerase High
技术资料
