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Recombinant Human Polymerase (

DNA directed), Lambda
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  • ¥1080 - 87906
  • Prospecbio
  • 以色列
  • enz-182
  • 2026年01月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      POLL

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      2ug/10ug/1mg

    规格:2ug产品价格:¥1080.0
    规格:10ug产品价格:¥2415.0
    规格:1mg产品价格:¥87906.0

    产品细节图片1

    CATALOGUE NUMBER

    ENZ-182

    SYNONYMS

    Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.

    INTRODUCTION

    DNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5’-deoxyribose-5-phosphate lyase activity which is synchronized by PCNA and replication protein A.

    DESCRIPTION

    POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.
    POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    SOURCE

    E.coli.

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    The POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 90% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    MG./S-SHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.
    POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    相关实验
    • Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis

      Site-directed mutagenesis permits modification of the functional characteristics of specific proteins and the characterization of regulatory DNA elements. Since the amplifying primers used in the polymerase chain reaction (PCR

    • Preparation of Phage Lambda DNA

      as vectors or for markers for gel electrophoresis can be obtained in this way. However, sometimes (for example, when screening recombinant phages) only small amounts of phage DNA are required. The purity of these samples needs only to be sufficient

    • Use of Polymerase Chain Reaction for Making Recombinant Constructs

      The capacity to recombine and modify DNA are underpinnings of the recombinant DNA revolution. The polymerase chain reaction (PCR) (1 ,2 ) provides a rapid means for the site-directed mutagenesis of DNA and for the recombination of DNA

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    文献支持
    Recombinant Human Polymerase (DNA directed), Lambda
    ¥1080 - 87906