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文献和实验Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis
Site-directed mutagenesis permits modification of the functional characteristics of specific proteins and the characterization of regulatory DNA elements. Since the amplifying primers used in the polymerase chain reaction (PCR
Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus
that is replicated by a rolling-circle mechanism (reviewed by refs. 1 and 2 ). There is no DNA intermediate. The infectious genomic strand is a template for an RNA-dependent RNA polymerase (most likely a modified activity of the host’s RNA polymerase II
Use of Polymerase Chain Reaction for Making Recombinant Constructs
The capacity to recombine and modify DNA are underpinnings of the recombinant DNA revolution. The polymerase chain reaction (PCR) (1 ,2 ) provides a rapid means for the site-directed mutagenesis of DNA and for the recombination of DNA
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