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Recombinant Moloney Mouse Leuk

emia Virus Reverse Trancscriptase
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  • ¥1080 - 10143
  • Prospecbio
  • 以色列
  • enz-310
  • 2026年01月05日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      MMLV RT

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      2,500U/10,000U/50,000U

    规格:2,500U产品价格:¥1080.0
    规格:10,000U产品价格:¥2415.0
    规格:50,000U产品价格:¥10143.0

    产品细节图片1

    CATALOGUE NUMBER

    ENZ-310

    DESCRIPTION

    MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).

    SOURCE

    Recombinant E. coli strain.

    PHYSICAL APPEARANCE

    Sterile Filtered clear solution (200 U/µl).

    FORMULATION

    50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.

    STABILITY

    Stable for 5 days at 10°C, for longer period of time store at -20°C.
    Please prevent freeze-thaw cycles.

    UNIT DEFINITION

    One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC.

    5X REACTION BUFFER

    250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).

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    文献支持
    Recombinant Moloney Mouse Leukemia Virus Reverse Trancscriptase
    ¥1080 - 10143