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Porcine Enteropeptidase/Entero

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  • ¥4830 - 23000
  • Prospecbio
  • 以色列
  • enz-267
  • 2026年01月01日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      pEnterokinase

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      100IU/500IU/1,000IU

    规格:100IU产品价格:¥4830.0
    规格:500IU产品价格:¥14500.0
    规格:1,000IU产品价格:¥23000.0

     

    CATALOGUE NUMBER

    ENZ-267

    SYNONYMS

    Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK, MGC133046.

    INTRODUCTION

    Enteropeptidase or enterokinase is an enzymeinvolved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberk?hn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen(a zymogen) to trypsin, indirectly activating a number of pancreaticdigestive enzymes.
    Enteropeptidase is a serine proteaseenzyme(EC3.4.21.9). Enteropeptidase is a part of the Chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.

    DESCRIPTION

    Porcine enteropeptidase is a specific protease which cleaves after the lysine at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave a site followed by proline. Theoretical Mw is 21,880 Dalton, the apparent Mw on SDS-PAGE is about 40 kDa.
    If a fusion tag is located in the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. ProSpec’s enterokinase is a highly purified enterokinase from porcine. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases.

    SOURCE

    Porcine.

    PHYSICAL APPEARANCE

    Sterile Liquid.

    FORMULATION

    2 IU/µl, 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

    STABILITY

    One year when stored at -20°C, one week at room temperature.

    UNIT DEFINITION

    One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Porcine enteropeptidase is a specific protease which cleaves after the lysine at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave a site followed by proline. Theoretical Mw is 21,880 Dalton, the apparent Mw on SDS-PAGE is about 40 kDa.

    相关实验
    • A Porcine Burn Model

      as well as in vitro preparations to study cutaneous burns. We have developed and validated a porcine model for partial-thickness contact burns, using it to successfully evaluate a variety of therapeutic agents (1 ). This chapter describes in detail the model

    • porcine brain tubulin prep

      . Solutions and reagents: 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L  0.1M HCl 6L  0.1 M NaOH 2L 0.1 M MgSO4 2L 10x Column Buffer ~10 L 1x column buffer 10 M NaOH 3 fresh ( Equipment: Waring Blender

    • Porcine brain tubulin preparation

      实验试剂   100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L 0.1M HCl 6L 0.1 M NaOH 2L 0.1 M MgSO4 2L 10x Column Buffer ~10 L 1x column buffer 10 M NaOH 300 ml

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    文献支持
    Porcine Enteropeptidase/Enterokinase
    ¥4830 - 23000