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Recombinant Bovine Enteropepti

dase/Enterokinase
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  • ¥1600 - 18500
  • Prospecbio
  • 以色列
  • enz-311
  • 2026年01月01日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      bEnterokinase

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      20IU/100IU/500IU

    规格:20IU产品价格:¥1600.0
    规格:100IU产品价格:¥5800.0
    规格:500IU产品价格:¥18500.0

     

    CATALOGUE NUMBER

    ENZ-311

    SYNONYMS

    Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK, MGC133046.

    INTRODUCTION

    Enteropeptidase or enterokinase is an enzyme involved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberk?hn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen (a zymogen) to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enteropeptidase is a serine protease enzyme (EC 3.4.21.9). Enteropeptidase is a part of the Chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.

    DESCRIPTION

    Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by E. Coli and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~28kDa.

    SOURCE

    E. Coli.

    PHYSICAL APPEARANCE

    Sterile liquid solution.

    FORMULATION

    Bovine EK in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

    STABILITY

    One year when stored at –20°C.
    Please avoid freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by SDS-PAGE.

    UNIT DEFINITION

    One unit is defined as the amount of enzyme needed to cleave 50µg of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by E. Coli and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~28kDa.

    相关实验
    • Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3

      adenoviruses an attractive alternative. Moreover, readministration of viral vectors is often required to maintain therapeutic levels of transgene expression, resulting in vector-specific immune responses. Although a number of features of bovine adenovirus (BAdV

    • The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes

      (1 ,2 ), as well as to look at the consequences of oncogene expression (3 ,4 ). Through this technique, recombinant DNA molecules of any type can be introduced into zygotes for use in producing transgenic domestic animals. Introduction of foreign genes into early embryos

    • DETECTION OF RECOMBINANT PROTEINS BY ELISA

      of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie

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    文献支持
    Recombinant Bovine Enteropeptidase/Enterokinase
    ¥1600 - 18500