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Recombinant Mouse Granulocyte

Macrophage-Colony Stimulating Factor
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  • ¥1080 - 46000
  • Prospecbio
  • 以色列
  • cyt-222
  • 2025年07月19日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      mGMCSF

    • 库存

      部分小规格有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/1mg

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:1mg产品价格:¥46000.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-222

    SYNONYMS

    CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, CSF2, GMCSF

    INTRODUCTION

    GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
    GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

    DESCRIPTION

    Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.
    GM-CSF Mouse is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    GM-CSF Mouse was lyophilized with no additives.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

    PROTEIN CONTENT

    GM-CSF quantitation was carried out by two independent methods
    1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

    2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.

    SAFETY DATA SHEET

    SDS

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    图标文献和实验
    该产品被引用文献

    5.Title:Pre-clinical Evaluation of a CEA DNA Prime/protein Boost Vaccination Strategy Against Colorectal Cancer.
    Publication:Article first published online: 21 JUN 2007 DOI:10.1111/j.1365-3083.2007.01945.x

     

    7. Title: O-glycosylated versus non-glycosylated MUC1-derived peptides as potential targets for cytotoxic immunotherapy of carcinoma.
    Publications: 
     Clinical & Experimental Immunology 143.1 (2006): 139-149.

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    文献支持
    Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor
    ¥1080 - 46000