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- 钦诚生物
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- QCB-1209-1
- 2025年07月09日
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一年
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钦诚生物
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50ml
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC
RNase and DEPC Treatment: Fact or Laboratory Myth
(0.1% and 1%). It would be impossible to predict the different interactions of DEPC with all molecular biology reagents. The most cautious approach for making RNase-free solutions would be to mix molecular biology grade powdered reagents up in DEPC-treated water. Alternatively
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