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- 详细信息
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX107681
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PKM
- 抗体英文名:
PKM antibody
- 抗体名:
PKM 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.
Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX107681) dilution: 1:1000
PKM antibody detects PKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PKM stained by PKM antibody (GTX107681) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: PKM stained by PKM antibody (GTX107681) diluted at 1:500.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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