- ￥1700 - 4000
- GeneTex
- 美国
- GTX102090
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX102090
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
- 浓度：
1.37 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377380)
- 抗体英文名：
NFkB p65 antibody
- 抗体名：
NFkB p65 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NFkB p65 antibody (GTX102090) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed U2OS, using NFkB p65(GTX102090) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Heng-Hsiung Wu et al., J Exp Med 2015 (PMID:25732306)
Yan T et al., FASEB J 2021 (PMID:34644426)
Liu PI et al., Aging (Albany NY) 2023 (PMID:36917086)
Li CJ et al., J Hepatocell Carcinoma 2023 (PMID:36741246)
Li Y et al., Exp Ther Med 2022 (PMID:35978933)
Ping SH et al., Iran J Basic Med Sci 2022 (PMID:36311191)
Zhang W et al., Oxid Med Cell Longev 2023 (PMID:36691640)
Cardile A et al., Int J Mol Sci 2023 (PMID:36674794)
Yu Fu et al., Evid Based Complement Alternat Med 2022 (PMID:35692570)
Manuela Leo et al., Antioxidants (Basel) 2022 (PMID:35453308)
Vichuda Charoensaensuk et al., Antioxidants (Basel) 2022 (PMID:35453424)
Xue Zhang et al., Front Pharmacol 2022 (PMID:35308231)
Wang Y et al., Aging (Albany NY) 2020 (PMID:33099539)
Li Y et al., Bioengineered 2022 (PMID:34964708)
Charoensaensuk V et al., Cells 2021 (PMID:34831265)
Lee KT et al., Aging (Albany NY) 2021 (PMID:34560673)
Su C.H. et al., Journal of Functional Foods 2021 86()
Hattori H et al., Mol Med Rep 2021 (PMID:34318909)
Zhang J et al., Mediators Inflamm 2021 (PMID:33958974)
Lin ZC et al., Theranostics 2021 (PMID:33754014)
Biswas S et al., Asian Pac J Cancer Prev 2021 (PMID:33773562)
Chang YX et al., Cancers (Basel) 2020 (PMID:33371405)
Chan HC et al., Biomedicines 2020 (PMID:33256187)
Zhu S et al., J Agric Food Chem 2020 (PMID:32981311)
Chung PC et al., Evid Based Complement Alternat Med 2020 (PMID:33014112)
Chang JW et al., Cereb Cortex 2020 (PMID:32248223)
Chao CC et al., Cancers (Basel) 2020 (PMID:32079335)
Chen CY et al., Front Oncol 2020 (PMID:32158695)
Gandhi KA et al., Free Radic Biol Med 2019 (PMID:31521664)
Wang Z et al., Int Immunopharmacol 2019 (PMID:31493665)
Li J et al., J Allergy Clin Immunol 2019 (PMID:31330222)
Li HY et al., J Agric Food Chem 2019 (PMID:30661353)
Li R et al., Int J Biol Macromol 2019 (PMID:30399378)
Leigh J et al., Can Urol Assoc J 2018 (PMID:30472982)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Arellano Buendia AS et al., Int J Mol Sci 2018 (PMID:30314265)
Chen ST et al., Oncogene 2018 (PMID:29993037)
Krga I et al., Free Radic Biol Med 2018 (PMID:29964169)
Gerzanich V et al., PLoS One 2018 (PMID:29617457)
Wang Y et al., Neural Plasticity 2018 2018()
Wang Y et al., Neural Plasticity 2018 (Epub)
Ricci C et al., Sci Rep 2018 (PMID:29396513)
Tsai CH et al., Sci Rep 2017 (PMID:29142311)
Chamard-Jovenin C et al., Front Endocrinol (Lausanne) 2017 (PMID:29109696)
Menicacci B et al., Int J Mol Sci 2017 (PMID:29084133)
Mahajan SD et al., Immunol Invest 2017 (PMID:29058550)
Sharma RK et al., Immunol Invest 2017 (PMID:29058549)
Wyss JC et al., Am J Physiol Renal Physiol 2017 (PMID:28971991)
Andreucci E et al., J Mol Med (Berl) 2017 (PMID:28929255)
Wang S et al., PLoS Genet 2013 (PMID:24039598)
Gerzanich V. et al., J Neuroinflammation 2017 (PMID:28865458)
Kuo CY et al., Cancer Res 2017 (PMID:28533271)
Huang YC et al., Toxicol Appl Pharmacol 2017 (PMID:28558962)
Liu MC et al., PLoS One 2017 (PMID:28558037)
Thiebaut C et al., PLoS One 2017 (PMID:28301550)
Leu JS et al., Am J Respir Crit Care Med 2016 (PMID:27858493)
Chen WH et al., Am J Sports Med 2016 (PMID:27400716)
Raninga PV et al., Cell Cycle 2016 (PMID:26743692)
F?ri I et al., PLoS One 2015 (PMID:26133168)
sakura042 本新手欲研究nfkb引起内皮细胞凋亡的机制：是否通过jnk通路。 这是否是一个信号通路串话的问题？ 我们用特异性抑制剂阻断nfkb后发现jnk活性下降，是否就能说明nfkb促进内皮细胞凋亡是通过jnk通路呢？还需要再阻断或者过表达jnk通路来反证吗？ 这个问题困扰了我好久，请教各位高手。 sakura042 自己顶一下 三叶虫 请问一下
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
人核转录因子p65(NF-kB p65)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人核转录因子p65 ( NF - kB p65 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 NF- kB p65单抗包被于酶标板上，标准品和样品中的 NF- kB p65 与单抗结合，加入生物素化的抗人NF- kB p65 ，形成免疫复合
技术资料暂无技术资料 索取技术资料
