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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102090
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
- 浓度:
1.37 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
IP/MS validation was supported by references (PMID:30377380)
- 抗体英文名:
NFkB p65 antibody
- 抗体名:
NFkB p65 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NFkB p65 antibody (GTX102090) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed U2OS, using NFkB p65(GTX102090) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Chen CY et al., Front Oncol 2020 (PMID:32158695)
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Li J et al., J Allergy Clin Immunol 2019 (PMID:31330222)
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Krga I et al., Free Radic Biol Med 2018 (PMID:29964169)
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Wang Y et al., Neural Plasticity 2018 2018()
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Tsai CH et al., Sci Rep 2017 (PMID:29142311)
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