- ￥1700 - 4000
- GeneTex
- 美国
- GTX107678
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP, EMSA
- Rabbit
- Human, Mouse, Rat
企业认证
相关产品推荐更多 >
![PTP1B antibody [001]](https://img1.dxycdn.com/2022/0328/078/9796577178128700453-14.jpg!wh200)
![CD168 / RHAMM antibody [N1N2], N-term](https://img1.dxycdn.com/2022/0328/839/1230766217404300453-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX107678
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, EMSA
- 浓度：
1.46 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377388)
- 抗体英文名：
NFkB p65 antibody
- 抗体名：
NFkB p65 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000 and competitor's antibody (#4764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Dhawan U et al., J Nanobiotechnology 2015 (PMID:26553043)
Chin YT et al., Mediators Inflamm 2016 (PMID:27504055)
Wang Y et al., Graefes Arch Clin Exp Ophthalmol 2016 (PMID:27405975)
Raninga PV et al., Oncotarget 2015 (PMID:25945832)
Chen T et al., Exp Dermatol 2014 (PMID:24758390)
Liu J et al., Arch Anim Nutr 2014 (PMID:24646150)
Liang CJ et al., Evidence-Based Complementary and Alternative Medicine 2014 (PMID:24723958)
Adrianto I et al., Nat Genet 2011 (PMID:21336280)
Hsieh JY et al., Stem Cells Dev 2010 (PMID:20367285)
Liang CJ et al., Free Radic Biol Med 2011 (PMID:21767632)
Pasula S et al., Front Genet 2022 (PMID:36199584)
Jhih-Ying Chi et al., Clin Transl Med. 2022 (PMID:36336784)
Xin Li et al., Transl Neurosci 2021 (PMID:33708438)
Qiu W et al., Environ Sci Technol 2022 (PMID:35286074)
Bankole O et al., Int J Mol Sci 2022 (PMID:35162978)
Chu J et al., Evid Based Complement Alternat Med 2020 (PMID:32695212)
Wu CS et al., Int J Immunopathol Pharmacol 2020 (PMID:32571120)
Shang S et al., Cancer Res 2019 (PMID:31439546)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Schiaffino L et al., Sci Rep 2018 (PMID:30150770)
Xiang W et al., Nat Commun 2018 (PMID:29968710)
Chang TM et al., Evidence-Based Complementary and Alternative Medicine 2017 2017()
Chen CC et al., PLoS One 2016 (PMID:27977759)
sakura042 本新手欲研究nfkb引起内皮细胞凋亡的机制：是否通过jnk通路。 这是否是一个信号通路串话的问题？ 我们用特异性抑制剂阻断nfkb后发现jnk活性下降，是否就能说明nfkb促进内皮细胞凋亡是通过jnk通路呢？还需要再阻断或者过表达jnk通路来反证吗？ 这个问题困扰了我好久，请教各位高手。 sakura042 自己顶一下 三叶虫 请问一下
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
人核转录因子p65(NF-kB p65)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人核转录因子p65 ( NF - kB p65 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 NF- kB p65单抗包被于酶标板上，标准品和样品中的 NF- kB p65 与单抗结合，加入生物素化的抗人NF- kB p65 ，形成免疫复合
技术资料暂无技术资料 索取技术资料
