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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG2a, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
Elab Fluor<sup>®</sup> 647
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F1045M
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
Elab Fluor® 647 Anti-Human CD20 Antibody[BCA/B20]_货号:E-AB-F1045M
- 抗体名:
Elab Fluor® 647 Anti-Human CD20 Antibody流式抗体[BCA/B20]_货号:E-AB-F1045M
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥2640.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥960.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥3960.0 |
货号:E-AB-F1045M
| Background |
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
|
| Alternate Names |
B-lymphocyte antigen CD20;MS4A1;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;MS4A1;CD20;
|
| Swissprot |
P11836
|
| Gene ID |
931
|
| Clone No |
BCA/B20
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
Elab Fluor® 647 Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
Elab Fluor<sup><sup>®</sup></sup> 647
|
| Conjugation Information |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验自2012年第一篇引用文献问世,至2023年8月,Elabscience已协助各地科研工作者发表超13,000篇SCI文献,包括行业顶/级期刊Nature Medicine, Nature, Cell, Signal Transduction and Targeted Therapy, Circulation, Molecular Cancer, Immunity, Advanced Materials等,总影响因子(IF)59472.59,其中IF≥30的文献超30篇,最高影响因子为66.849,发文单位包含北京大学、中国科学技术大学、浙江大学、斯坦福大学、耶鲁大学、柏林夏里特大学等国内外各大高校。
每年引用文献数量持续增长,保持逐年30%+高增长率,可以预见,Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
文献详情查看>>>>


membrane, creating a structure similar to an ion channel Delivers early signal in B cell activation, allowing resting B cells to respond to later antigens Rituximab is a chimeric murine-human anti-CD20 antibody used for treatment of B cell
挑选14个克隆进行筛选,结果发现14个克隆均能与靶抗原有反应,其中以4(E4F4)、5(E5F5)、6(G4)、7(G5)、11(G9)克隆颜色较深,挑取6号克隆,利用APAAP法进一步检测其结合活性。结果显示,克隆6的表达产物能和Daudi细胞特异结合。克隆6的测序结果和氨基酸序列分析表明该基因序列完全符合PDB库中抗体所具有的若干保守的框架氨基酸的特点,该序列是一抗体基因序列。2、抗CD20嵌合抗体Fab和F(ab)2的表达与纯化SDS-PAGE电泳结果表明Protein G可有效纯化Fab
Immune Response Biomarker Profiling
a total of 5 wash steps. 8) During the wash steps, mix 2.5 L Alexa Fluor® 647 goat anti-human IgG antibody with 5 mL Washing Buffer per array to obtain a final antibody concentration of 1 g/mL. Store on ice until use. Optional: add Alexa Fluor®®
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