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- Elabscience
- 湖北武汉
- E-AB-F1029D
- 2025年07月12日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rat IgG2a, κ
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1029D
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 抗体英文名：
PE Anti-Mouse CD122/IL-2RB Antibody[5H4]_货号:E-AB-F1029D
- 抗体名：
PE Anti-Mouse CD122/IL-2RB Antibody流式抗体[5H4]_货号:E-AB-F1029D
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥684.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥384.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1200.0 |
货号：E-AB-F1029D
| Background |
CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.
|
| Alternate Names |
Interleukin-2 receptor subunit beta;Il2rb;IL-2 receptor subunit beta; IL-2R subunit beta;IL-2RB;High affinity IL-2 receptor subunit beta;p70-75;CD122;
|
| Swissprot |
P16297
|
| Gene ID |
16185
|
| Clone No |
5H4
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
., Schulz, H., Wolf, E., Wurst, W., Zimmer, A., Busch, D.H., and Hradě de Angelis, M. 2005. Introducing the German Mouse Clinic: Open access platform for standardized phenotyping. Nat. Methods 2:403‐404.
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