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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX105620
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC
- 浓度:
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
AKR1C1
- 抗体英文名:
AKR1C1 antibody
- 抗体名:
AKR1C1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GTX105620) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX105620 diluted at 1:1000
AKR1C1 antibody detects AKR1C1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
AKR1C1 antibody (GTX105620) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GTX105620) antibody at 1:200 dilution.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Jiang Y et al., Br J Haematol 2020 (PMID:31943135)
Zeng C et al., Front Physiol 2019 (PMID:31333491)
Chenming Zeng et al., Front. Physiol. 2019 (Epub)
ZHU H et al., Theranostics 2018;8(3)
Nomura M et al., J Clin Bioinforma 2011 (PMID:21888658)
Lin-Lin Chang et al., Transl Oncol 2022 (PMID:35429904)
Nadeem L et al., J Cell Mol Med 2021 (PMID:34114342)
Marino N et al., NPJ Breast Cancer 2020 (PMID:33083529)
Gupta R et al., Endocrinology 2017 (PMID:28531318)
Zhu H et al., Theranostics 2018 (PMID:29344298)
Zhu H et al., Theranostics 2020 (PMID:32104503)
Generation of Antibody Molecules Through Antibody Engineering
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General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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