- ￥1700 - 4000
- GeneTex
- 美国
- GTX101794
- 2025年07月16日
- WB, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat, Cat, Dog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Cat, Dog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101794
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, IHC-Fr
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Actin Gamma 1
- 抗体英文名：
Actin Gamma 1 antibody
- 抗体名：
Actin Gamma 1 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Actin Gamma 1 antibody detects Actin Gamma 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
Actin Gamma 1 stained by Actin Gamma 1 antibody (GTX101794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody (GTX101794) diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin(GTX101794) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101794 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX101794 diluted at 1:1000
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文献和实验Xie Y et al., Anal Biochem 2019 (PMID:30981700)
Li W et al., J Diabetes Res 2018 (PMID:29955616)
Chen YH et al., Cancers (Basel) 2019 (PMID:31430901)
Chang YC et al., Cancer Res 2019 (PMID:31358528)
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
大鼠Actin-b(Actin-b)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Actin- b (Actin- b )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Actin- b 单抗包被于酶标板上，标准品和样品中的 Actin- b 与单抗结合，加入生物素化的抗大
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
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